The call between the leaders came after Palestinian health officials reported at least 50 Palestinians killed by Israeli airstrikes over a 24-hour period.
Biden stressed to Netanyahu "the urgency of bringing the ceasefire and hostage release deal to closure and discussed upcoming talks in Cairo to remove any remaining obstacles," a White House statement said.
But Israel and Hamas on Wednesday held to their demands. Netanyahu's office said in a statement that Israel had not agreed to drop its demand to maintain troops in the Philadelphi Corridor, the border strip between Gaza and Egypt, an issue that has been a major sticking point.
A Hamas statement reiterated key Hamas demands, including an end to Israel's Gaza operation, a full Israeli pullout and a deal to exchange Israeli hostages for Palestinian prisoners held by Israel.